rapid purification of hu protein from halobacillus karajensis

نویسندگان

parinaz ghadam

rana samadi

چکیده

the histone-like protein hu is the most-abundant dna-binding protein in bacteria. the hu protein non-specifically binds and bends dna as a hetero- or homodimer, and can participate in dna supercoiling and dna condensation. it also takes part in dna functions such as replication, recombination, and repair. hu does not recognize any specific sequences but shows a certain degree of specificity to cruciform dna and repair intermediates such as nick, gap, bulge, etc. to understand the features of hu binding to dna and repair intermediates, a fast and easy hu protein purification method is required. here we report a two-step purification procedure of hu from halobacillus karajensis (the gram positive and moderately halophilic bacteria isolated from karaj surface soil). the method of hu purification allows obtaining a pure non-tagged protein. salting out and ion exchange chromatography were applied for purification, and the purified protein was identified by immunoblotting. results showed that the molecular weight of the purified protein was approximately 11 kda which is immunologically similar to the bacillus subtilis hu protein (hbsu).

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Rapid purification of HU protein from Halobacillus karajensis

The histone-like protein HU is the most-abundant DNA-binding protein in bacteria. The HU protein non-specifically binds and bends DNA as a hetero- or homodimer, and can participate in DNA supercoiling and DNA condensation. It also takes part in DNA functions such as replication, recombination, and repair. HU does not recognize any specific sequences but shows a certain degree of specificity to ...

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Rapid purification of HU protein from Halobacillus karajensis

The histone-like protein HU is the most-abundant DNA-binding protein in bacteria. The HU protein non-specifically binds and bends DNA as a hetero- or homodimer, and can participate in DNA supercoiling and DNA condensation. It also takes part in DNA functions such as replication, recombination, and repair. HU does not recognize any specific sequences but shows a certain degree of specificity to ...

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استخراج و تخلیص پروتئین hu از باکتری halobacillus karajensis

میکروارگانیسم ها برای فشرده کردن dna سلولی از گروهی از پروتئین های بازی استفاده می کنند. این گروه از پروتئین ها اثرات عمیقی بر روی بیان ژن ها، نوترکیبی، همانندسازی dna، رشد و توانایی حیات دارند و به عنوان پروتئین های شبه هیستونی شناخته می شوند. از جمله ی این پروتئین ها می توان به hu، h-ns، fis و اشاره کرد. پروتئین های hu برای نگهداری ساختار نوکلئوئید ضروری هستند. بعلاوه آنها در وظایف وابسته ب...

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Halobacillus karajensis sp. nov., a novel moderate halophile.

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عنوان ژورنال:
molecular biology research communications

ناشر: shiraz university press

ISSN 2322-181X

دوره 3

شماره 1 2014

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